Fig. 4.

Bovine serum albumin digestion in gastric phase of a model gut system with and without SP54. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 0.5 g BSA was digested alone (control digestion) and in the presence of varying concentrations of SP54. Control digestion is represented as (■) and digestion with SP54 at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. At T_[60] by the end of the gastric phase, 125, 250 and 500 mg of pentosan polysulphate significantly inhibited the gastric digestion of protein by 54.1% (P = 0.0001), 78.9% (P = 0.001) and 87.6% (P = 0.0004), respectively (t-test).