Table 1.
Summary of transfection efficiency and IgM binding of TM284var1 DBL4ζ deletion constructs expressed in COS-7 cells.
| Construct | Cysteines | Transfection efficiencya (%) | IgM bindingb (%) |
|---|---|---|---|
| 1c E1481-T1952 | C1–C16 | 10–15 | 8–12 |
| 2 K1595-H1839 | C4–C11 | 15–20 | 10–15 |
| 3 K1595-E1814 | C4–C10 | 25–30 | 10–15 |
| 4 K1595-L1799 | C4–C9 | 20 | 0 |
| 5 K1595-E1773 | C4–C8 | 15–20 | 0d |
| 6 P1615-H1839 | C6–C11 | 10–15 | 0d |
| 7 P1615-E1814 | C6–C10 | 10–15 | 0 |
| 8 Q1625-H1839 | C7–C11 | 15–20 | 0 |
a
Transfection efficiency was determined by counting the percentage of COS-7 cells showing surface fluorescence by IFA with mAb DL6.
b
IgM binding was determined by counting the percentage of COS-7 cells showing surface fluorescence by IFA using a mouse anti-human IgM mAb. Data from at least 2 experiments for each construct is shown.
c
Construct used in previous study [2].
d
Some faint positive cells (1–2%) were seen with these constructs in some but not all experiments (n = at least 3).