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. 2015 Apr 15;51(7):714–721. doi: 10.1007/s11626-015-9885-z

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Figure 4. — Colony formation assay. (a) Primary WT mesothelial cells were able to form colonies in plates with soft agar, as well as the human MM-derived cell line MSTO-211H (b) used as a positive control (derived from a biphasic mesothelioma). Selected colonies typically representing the morphological appearance of immortalized cells iMeso-WT1 (c), and iMeso-NF3 (d) are shown. Scale bar: 250 μm.