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. 2015 Aug 17;35(18):3163–3177. doi: 10.1128/MCB.00118-15

FIG 4.

FIG 4

GRHL1-dependent genes activated in trophoblast cells differentiating in vitro. (A) qRT-PCR analyses of Grhl1, Grhl2, and Grhl3 transcripts in stem (Stem) and differentiated (Diff) Rcho-1 TS cells. (B) Western blot analysis of GRHL1 and GAPDH expression in stem and differentiated Rcho-1 TS cells. (C and D) GRHL1 knockdown efficiency in differentiated Rcho-1 TS cells expressing control (Ctrl) or Grhl1 shRNA was validated by qRT-PCR (C) and Western blot (D) analyses. (E) Analysis of trophoblast invasive abilities in differentiated Rcho-1 TS cells expressing control or Grhl1 shRNAs by Matrigel transwell chamber assays. (F) qRT-PCR analysis of Prl3d1, Crispld2, Cgm4, Cyp11a1, Ppap2b, and Mmp9 transcripts in differentiated Rcho-1 TS cells expressing control or Grhl1 shRNAs. Bars in the histograms in panels A, C, E, and F represent means ± SEM. Values significantly different from those of controls are indicated with an asterisk (P < 0.05).