FIG 5.

Association of FOSL1 with JUN family members in trophoblast cells. (A) Western blot analysis of AP-1 family proteins using nuclear extracts from stem (Stem) or differentiated (Diff) Rcho-1 TS cells. Lysates from 293FT cells overexpressing JUND were used as a positive control (PC) for JUND expression. (B) Coimmunoprecipitation analysis for detection of FOSL1 binding partners. (Left) Immunoprecipitation (IP) using IgG or antibodies to JUN or JUNB and Western blotting for FOSL1. (Right) Immunoprecipitation using IgG or antibodies to FOSL1 and Western blotting for JUN or JUNB. (C) Immunohistochemical characterization of FOSL1 and JUN or of FOSL1 and JUNB on the same sections of gestation day 13.5 rat placenta. (D) Immunohistochemical characterization of FOSL1 and JUND on serial sections of gestation day 13.5 rat placenta. Nuclei were visualized with DAPI. Bar = 100 μm.