TABLE 2.
Specific primer sequences used for qRT-PCR analysis
| Target gene | Forward primer | Reverse primer | GenBank accession no. |
|---|---|---|---|
| 18s | GCAATTATTCCCCATGAACG | GGCCTCACTAAACCATCCAA | NR_046237.1 |
| Fosl1 | ATCCCCGACCTCTGACCTAT | CAAGGCGTTCCTTCTGCTT | NM_012953.1 |
| Prl3d1 | TTCGGGCTCTGGTATGCAAC | TGGACACAATGGCAGTTGGTTTGG | NM_017363.3 |
| Prl5a1 | TCCACACCAGACATTCCAGA | TTTCCAGGAAGCCAACATTC | NM_138527.1 |
| Gcm1 | CCCCAACAGGTTCCACTAGA | AGGGGAGTGGTACGTGACAG | NM_017186.2 |
| Pcdh12 | GAGCCTGGTTCGACTCTCTG | GGGCTTGGCCGAGTATTTAT | NM_053944.1 |
| Crispld2 | GTATCCCCCTGCCTCCAAC | CAGTGCACAGCCAGGTTCT | NM_138518.2 |
| Grhl1 | AAGGGATCCACCACCCTATC | ATGAAGACCTTCGCCTCATC | XM_234006.8 |
| Cgm4 | GTTCCCTGTGTATCCGCAAT | GTGAGCTTGGCAGGGTTAAA | NM_012525.1 |
| Cyp11a1 | ACAAGCTGCCCTTCAAGAAC | CGCAGCATCTCCTGTACCTT | NM_017286.2 |
| Ppap2b | GCCTCCTTCTCCATGTTCAC | AAGGCCATCATGAGCAAAGT | NM_138905.2 |
| Mmp9 | AACTTCGACGCTGACAAGAA | TTTAGAGCCACGACCATACA | NM_031055.1 |
| Jun | TAACAGTGGGTGCCAACTCA | CGCAACCAGTCAAGTTCTCA | NM_021835.3 |
| Junb | CAGTTACTCCCCAGCCTCTG | ATGTGGGAGGTAGCTGATGG | NM_021836.2 |
| Jund | TGTTGCGTGTGTGTTTCCTT | CCAAGGATTACGGAACAGGA | NM_138875.4 |
| Ddx60 | GTCCTAAGCCAAAGAAGGAT | TGCCCTTCTTTTTGTTCTTCC | XM_008771220.1 |
| Mfap5 | AGAAGGCCTTGCTGCTTGT | CCGCCAGAACTGTATCGTCT | NM_001108644.1 |
| Rdh12 | CTCTTCTCGCCCTTCTTCAA | GCGCTCAGCTGTTTTCTTGT | NM_001108037.1 |
| Micall2 | GACGTGAGCATCACCAACAT | AGCTGTTCCTCAGCCACTTG | XM_006248975.2 |
| Il1r2 | CTCCCCTGGAGACAATACCA | TGGTGTTGGAAGATGTACCAGT | NM_053953.1 |
| Mmd | GATTTTCTCCAGCCTTGGTG | AATGATGCCATCGCTCTTG | NM_001007673.1 |
| LOC171573 | TGAGGCTAAAGAGGACCAGG | CTGACATGCTCCATTGCCAG | NM_138537.2 |
| Flt1 | GGGTGTCTGCTTCTCACAGG | TTTTTGTCCTCCTGGCTCAC | NM_019306.1 |
| Hspb8 | GAAGGTGGGATTGTCTCCAA | CCTGGTTGTCTTGAGGAAGC | NM_053612.2 |
| Ccdc125 | AAAGCCACAAGCCACACAC | ATTCCATTTCCCACTGCAAG | NM_001134761.1 |
| Fdxr | AGATCTGTGTGGTCGGCAGT | AGCGGGCTGTCTGTGTAAAT | NM_024153.1 |
| Rilpl1 | AGACCAAGGAGCAGGAGATG | GGTTGGGGTCCTTGAGGT | NM_001191665.1 |
| Ceacam9 | CCTGCTGATCAGAAACGTCA | AGGTGAGGGTCACAGAACCA | NM_053919.2 |
| Chdh | GTTGGGAATGCAGATGACCT | ACCTCCAGAGCCACTCCAG | NM_198731.2 |
| Ceacam3 | TGGTACAAAGGGCTGACAAA | TCCACAGGTCAAAGTGGAGAA | NM_012702.2 |
| Sema6d | GGCCAGTGATGCTGTCATTT | TATGTTCCACGGCGATTTCT | NM_001107768.1 |
| Cd47 | GAAAAACCGCCCAGTTTCGTGGT | CGGCAACCAGCAGCAGAATGA | NM_019195.2 |
| Cd9 | GCATGCTGGGATTGTTCTTCGGAT | ATGGATGGCTTTGAGTGTTTCCCG | NM_053018.1 |
| Il17f | CAAAACCAGGGCATTTCTGT | GACCAGGATTTCTTGCTGGA | NM_001015011.1 |
| Prl4a1 | TCTGTCTTTGTCTCACCAATGG | GTTCAAACCCTCGGCCTTG | NM_017036.2 |
| Dio3 | CCGCATATGGTGCCTATTTT | GCCCACCAATTCAGTCACTT | NM_017210.3 |
| Mt1a | CACCAGATCTCGGAATGGAC | GTTCGTCACTTCAGGCACAG | NM_138826.4 |
| Adm | TCATCGCAGTCAGTCTTGGA | ACGACTTAGCGCCCACTTAT | NM_012715.1 |
| Zfp36l2 | TTGCAGTTCCGACCATTACA | AACCCCGGAGTGAAGCTC | NM_001036626.1 |
| Ptprk | TTTGAGTGGGTCCATGTCAG | CCTTCTGGCTGTACAACAGGT | NM_001029902.2 |
| Tex19.2 | TTTTGGCCTCATGGGAGATA | CAGCTTCACACTTGCTCCAA | NM_001109622.1 |
| Ppap2a | ACCATTTACAAAGCCGTTGG | AGTGCCCCGAGTAGAAGGAC | NM_022538.2 |
| Ceacam1 | ACCCAATCAAGCTGGACGTA | TTGAGGGTTTGTGCTCTGTG | NM_031755.2 |
| Igdcc3 | TGAGGGCGACTATGAATGTG | TGGGGACTCTGTTCTTCTCC | XM_006243296.2 |
| Akap12 | GCAGGAGGAGACATCAGAAA | CTTCGCCTTCATCCTTCTTG | NM_057103.2 |
| Icam2 | TTTCACTTGTTCGGGAAAGC | TCTGCCACAGAGCAGAGAGA | NM_001007725.1 |
| Rbks | GCTTGCCAAAAACTGGAGAA | GGAGGCTGTTCCTGTAGCTG | NM_001108703.1 |
| Myo1f | CCAAGAACCCTCTGAACCTG | GATGGCGTCCTCAGTGATCT | NM_001108076.1 |
| Chchd10 | CTCAGCTGTAGGGCATGTCA | GAAGCCCTCACACAGGGTTA | NM_001007008.1 |
| Gbx2 | GACGGCAAAGCCTTCTTG | CGCTCTCCAGAGAGAAGCTC | NM_053708.2 |
| H19 | AGCTCGGACTGGAGACTAGG | GGCAAAGGAAAGAACAGACG | NR_027324.1 |
| Bex1 | AGCAGGAGGAGGAGGAAGAG | TCCTCCTTTTTCTGATGGTCA | NM_001037365.1 |
| Serpinh1 | CCAGAGGTCACCAAGGATGT | GGTGCATCATCGTAACACCC | NM_017173.1 |
| Mmp12 | GCACATTTTGATGAGGCAGA | TTGATTTTGGATTATTGGAATGC | NM_053963.2 |
| Il11ra1 | CAAGTTCCGGTTGCAATACC | TAGGAGTACCCCAGGCCTCT | NM_139116.1 |
| Grhl2 | TGGAAGCCACCAAATCTCTC | GGTTTATTGCTGCGGTTGTT | NM_001134527.1 |
| Grhl3 | GAGACCCAGCCTGTGTTGTT | CCTCGCTTGCATTTCTTGTA | NM_001106690.1 |