TABLE 5.

Selected genes downregulated by Fosl1 shRNA in differentiated Rcho-1 TS cells identified by microarray analysis and validated by qRT-PCR analysis

Protein	Gene symbol	Functiona	Fosl1 knockdown/control knockdown transcript expression ratio by:
			Microarray	qRT-PCR (±SEM)
DEAD (Asp-Glu-Ala-Asp) box polypeptide 60	Ddx60	RNA helicase	0.04	0.17 ± 0.02
Microfibrillar-associated protein 5	Mfap5	Cell adhesion/ECM	0.07	0.37 ± 0.11
Retinol dehydrogenase 12	Rdh12	Retinoid metabolism	0.12	0.17 ± 0.02
Prolactin family 3, subfamily d, member 1	Prl3d1	Hormone/cytokine	0.13	0.27 ± 0.04
Cys-rich secretory protein LCCL domain 2	Crispld2	ECM assembly	0.17	0.28 ± 0.04
Mical-like 2	Micall2	Cytoskeleton organizer	0.20	0.17 ± 0.02
Interleukin 1 receptor type II	Il1r2	Decoy cytokine receptor	0.20	0.09 ± 0.02
Monocyte-to-macrophage differentiated	Mmd	Progestin/adipoq receptor family, unknown function	0.20	0.20 ± 0.03
Grainyhead-like 1	Grhl1	Transcription factor	0.21	0.25 ± 0.04
Carcinoembryonic antigen gene family 4	Cgm4	Ligand; immune/vasculature	0.21	0.37 ± 0.05
Cytochrome P450, family 11, subfamily a, polypeptide 1	Cyp11a1	Steroid biosynthesis	0.25	0.32 ± 0.05
Spleen protein 1 precursor	LOC171573	Unknown function	0.27	0.17 ± 0.03
Fms-related tyrosine kinase 1	Flt1	Growth factor receptor; vasculature	0.28	0.29 ± 0.04
Heat shock protein B8	Hspb8	Chaperone; stress response	0.30	0.36 ± 0.05
Coiled-coil domain-containing 125	Ccdc125	Cell motility	0.32	0.52 ± 0.10
Phosphatidic acid phosphatase 2B (LPP3)	Ppap2b	Phospholipid phosphatase; signal transduction	0.34	0.32 ± 0.05
Ferredoxin reductase	Fdxr	Mitochondrial flavoprotein; electron transport	0.34	0.66 ± 0.05
Rab-interacting lysosomal protein-like 1	Rilpl1	Regulation of cilium function	0.36	0.57 ± 0.09
Carcinoembryonic antigen-cell adhesion molecule 9	Ceacam9	Cell adhesion	0.36	0.34 ± 0.04
Choline dehydrogenase	Chdh	Mitochondrial phospholipid and 1-carbon metabolism	0.36	0.36 ± 0.05
Matrix metalloproteinase 9	Mmp9	ECM remodeling	0.38	0.50 ± 0.08
Carcinoembryonic antigen-related cell adhesion molecule 3	Ceacam3	Cell adhesion	0.40	0.13 ± 0.03
Semaphorin 6D	Sema6d	Ligand; cell guidance	0.41	0.71 ± 0.14
Cd47 molecule	Cd47	Cell adhesion/thrombospondin receptor	0.44	0.55 ± 0.07
Cd9 molecule	Cd9	Tetraspanin; cell adhesion/signal transduction	0.55	0.59 ± 0.08
Interleukin 17F	Il17f	Cytokine	0.66	0.64 ± 0.17
Prolactin family 4, subfamily a, member 1	Prl4a1	Hormone/cytokine	0.66	0.34 ± 0.06

^aECM, extracellular matrix.