TABLE 6.
Selected genes upregulated by Fosl1 shRNA in differentiated Rcho-1 TS cells identified by microarray and validated by qRT-PCR analysis
| Protein | Gene symbol | Functiona |
Fosl1 knockdown/control knockdown transcript expression ratio by: |
|
|---|---|---|---|---|
| Microarray | qRT-PCR (±SEM) | |||
| Deiodinase, iodothyronine, type iii | Dio3 | Thyroid hormone inactivation | 3.52 | 2.86 ± 0.23 |
| Metallothionein 1a | Mt1a | Metal ion homeostasis | 2.97 | 5.85 ± 0.42 |
| Adrenomedullin | Adm | Ligand; vasculature | 2.58 | 2.20 ± 0.43 |
| Zinc finger protein 36, c3h-type-like 2 | Zfp36l2 | Putative transcription factor | 2.45 | 2.46 ± 0.27 |
| Protein tyrosine phosphatase, receptor type, k | Ptprk | Protein tyrosine phosphatase; signal transduction | 2.36 | 2.77 ± 0.25 |
| Testis-expressed gene 19.2 protein | Tex19.2 | Unknown function | 2.30 | 1.72 ± 0.54 |
| Phosphatidic acid phosphatase type 2a | Ppap2a | Phospholipid phosphatase; signal transduction | 2.21 | 3.17 ± 0.09 |
| Carcinoembryonic antigen-related cell adhesion molecule 1 | Ceacam1 | Cell adhesion | 2.16 | 4.36 ± 0.37 |
| Immunoglobulin superfamily, dcc subclass, member 3 | Igdcc3 | Unknown function | 2.15 | 2.52 ± 0.13 |
| A kinase (Prka) anchor protein 12 | Akap12 | Scaffold protein for protein kinase A/C; signal transduction | 2.13 | 1.92 ± 0.20 |
| Intercellular adhesion molecule 2 | Icam2 | Cell adhesion; leukocytes, immune function | 2.11 | 1.85 ± 0.19 |
| Ribokinase | Rbks | Ribose metabolism; energy production, nucleotide and amino acid biosynthesis | 2.09 | 3.32 ± 0.05 |
| Myosin IF | Myo1f | Unconventional myosin; intracellular movement | 2.08 | 3.35 ± 0.12 |
| Coiled-coil-helix-coiled-coil-helix domain-containing 10 | Chchd10 | Mitochondrial protein, possibly oxidative phosphorylation | 2.08 | 2.30 ± 0.09 |
| Gastrulation brain homeobox 2 | Gbx2 | Transcription factor | 1.97 | 2.89 ± 0.62 |
| H19, imprinted maternally expressed transcript | H19 | Noncoding RNA; imprinting | 1.94 | 2.25 ± 0.09 |
| Brain-expressed gene 1 protein | Bex1 | Adaptor; signal transduction | 1.92 | 1.99 ± 0.21 |
| Serine (or cysteine) peptidase inhibitor, clade h, member 1 | Serpinh1 | Collagen-specific chaperone; collagen biosynthesis | 1.89 | 1.73 ± 0.29 |
| Matrix metallopeptidase 12 | Mmp12 | ECM remodeling | 1.76 | 1.89 ± 0.64 |
| Interleukin 11 receptor, alpha chain 1 | Il11ra1 | Cytokine receptor-associated signal transducer | 1.73 | 1.85 ± 0.26 |
a
ECM, extracellular matrix.