FIG 1.

XPG is required for EGF-induced FOS transcription. (A) Immunoblots of whole-cell extracts from wild-type HeLa cells (HeLa WT), or HeLa cells stably expressing short hairpin RNA against luciferase (ctrl KD) or XPG (KD #1 and KD #2), probed with the antibodies indicated on the left. (B) Real-time PCR analysis for FOS and GAPDH mRNAs. Total RNA was prepared from cells stimulated for the indicated times with 0.1 μg/ml EGF. The results are normalized to the unstimulated condition at time zero and expressed as the mean ± standard error of the mean (SEM) from at least three independent experiments. (C) Real-time PCR analysis of EGF-inducible and housekeeping gene expression. Total RNA prepared from cells stimulated for 30 min with 0.1 μg/ml EGF was examined as described for panel B. (D) XPG knockdown has little effect on the EGF signal transduction pathway. Whole extracts from cells that were stimulated for 7.5 min with 0.1 μg/ml EGF or left untreated (−) were analyzed by immunoblotting with the antibodies indicated on the left.