Table 2.
Day 6 in vitro implanted mouse embryos were exposed (at 37°C, 5% CO2 in air) for 48 hours to either (1) control medium with heat-inactivated HPV-18 HeLa lysate, (2) HPV-18 HeLa lysate, (3) HPV-18 HeLa lysate and 0.5 µM selenomethionine (SeMet), or (4) HPV-18 HeLa lysate and 5.0 µM SeMet. The percentages of live, apoptotic, and necrotic trophoblast cells were assessed using bisbenzimide and propidium iodide dual-stain epifluorescence analyses.
| Trophoblast cells group | Total cells (n) | Number of viable cells (%) | Number of apoptotic cells (%) | Number of necrotic cells (%) | Number of total nonviable cells (%) |
|---|---|---|---|---|---|
| (1) Control group | 64 | 17 (23.6) | 47 (76.4) | 0 (0) | 47 (76.4) |
| (2) HPV-18 HeLa lysate | 134 | 16 (13.3)a | 109 (79.8) | 9 (6.9)a | 118 (86.7)a |
| (3) HPV-18 HeLa and 0.5 µM SeMet | 187 | 51 (24.2)b | 111 (62.2)b | 25 (13.6)a,b | 136 (75.8)b |
| (4) HPV-18 HeLa lysate and 5.0 µM SeMet | 132 | 35 (24.0)b | 74 (58.7)b | 23 (17.4)a,b | 97 (76.1)a,b |
aDifferent from the control (1) group (p < 0.05).
bDifferent from the HPV-18 HeLa lysate (2) group (p < 0.05).