Table 3.
Day 6 in vitro implanted mouse embryos were exposed (at 37°C, 5% CO2 in air) for 48 hours to either (1) control medium with heat-inactivated HPV-18 HeLa lysate, (2) HPV-18 HeLa lysate, (3) HPV-18 HeLa lysate and 0.5 µM selenomethionine (SeMet), or (4) HPV-18 HeLa lysate and 5.0 µM SeMet. The percentages of live, apoptotic, and necrotic inner cell mass (ICM) cells were assessed using bisbenzimide and propidium iodide dual-stain epifluorescence analyses.
| Treated ICM cells groups | Total cells (n) | Number of viable cells (%) | Number of apoptotic cells (%) | Number of necrotic cells (%) | Number of total nonviable cells (%) |
|---|---|---|---|---|---|
| (1) Control group | 71 | 32 (45.5) | 38 (53.2) | 1 (1.3) | 39 (54.5) |
| (2) HPV-18 HeLa lysate | 365 | 41 (17.5)a | 236 (55.3) | 88 (27.2)a | 324 (82.5)a |
| (3) HPV-18 HeLa and 0.5 µM SeMet | 236 | 44 (19.2)a | 123 (50.6) | 69 (30.1)a | 192 (80.8)a |
| (4) HPV-18 HeLa lysate and 5.0 µM SeMet | 210 | 36 (20.6)a | 148 (68.0) | 26 (11.4)a,b | 174 (79.4)a |
aDifferent from the control (1) group (p < 0.05).
bDifferent from the HPV-18 HeLa lysate (2) group (p < 0.05).