Table 3.
Overall ranking of the candidate reference genes for cell cultures and for seedlings after geNorm, NormFinder, and BestKeeper analysis.
| Cell cultures |
Seedlings |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene | geNorm rank | Normfinder rank | BestKeeper rank | Total score | Overall rank | geNorm rank | Normfinder rank | BestKeeper rank | Total score | Overall rank |
| TIP41 | 3 | 2 | 3 | 8 | 1 | 4 | 6 | 6 | 16 | 4 |
| PP2AA2 | 2 | 1 | 9 | 12 | 2 | 3 | 3 | 5 | 11 | 3 |
| UBC | 4 | 3 | 5 | 12 | 2 | 5 | 4 | 8 | 17 | 5 |
| SAND | 5 | 4 | 4 | 13 | 3 | 2 | 2 | 3 | 7 | 2 |
| PP2AA3 | 1 | 9 | 6 | 16 | 4 | 7 | 7 | 7 | 21 | 6 |
| CYP5 | 8 | 7 | 2 | 17 | 5 | 6 | 5 | 10 | 21 | 6 |
| UBQ10 | 9 | 10 | 1 | 20 | 6 | 9 | 8 | 4 | 21 | 6 |
| ACT2 | 6 | 5 | 10 | 21 | 7 | 11 | 11 | / | 33 | / |
| Clath | 7 | 8 | 8 | 23 | 8 | 1 | 1 | 1 | 3 | 1 |
| PROF | 10 | 6 | 7 | 23 | 8 | 8 | 9 | 9 | 26 | 8 |
| βTUB | 11 | 11 | / | 33 | / | 10 | 10 | 2 | 22 | 7 |
| ACT7 | 12 | 12 | / | 36 | / | 12 | 12 | / | 35 | / |
The two lowest ranked genes according to geNorm and Normfinder were excluded from the BestKeeper analysis.