FIGURE 1.

Comprehensive microarray polymer profiling (ComPP) of wild type (NPB) and mutant lines cslf6-1 and cslf6-2. Rice cell wall material was sequentially extracted with CDTA followed by NaOH. The extracts were then subsequently printed on nitrocellulose membranes and probed with a library of cell wall specific monoclonal antibodies to determine relative abundance. In our experiment, the (1–3), (1–4)-β-D-glucan antibody labeling (MLG) from NaOH extracted wild type (NPB) samples had the highest fluorescent count and the array was normalized accordingly in reference to the MLG signal. ComPP analysis showed significant difference in only MLG (boxed).