Figure 1.

Transient expression of P. parasitica PpPDI1 induces strong cell death on N. benthamiana. (A) Infiltration of Agrobacterium tumefaciens strain AGL1 carrying PpPDI1 construct into leaves of 6-week-old N. benthamiana plants, compared with the control construct GFP. The necrotic responses were scored and photographs were taken 3 days post infiltration (dpi). (B) Functional domains of PpPDI1 protein. PpPDI1 shows four TRX domains and two catalytic domains containing characteristic CGHC active-site motif. (C) Alignment of the active domains (a and a′) of P. parasitica PpPDI1 with PDI proteins of different organisms using the Clustal X program. Identical amino acid residues are highlighted against black background shading; similar amino acid residues are shaded with a light gray background, and distant similar amino acids are not shaded. The sequences for active motifs of CGHC are boxed.