Skip to main content
. 2015 Aug 18;6:632. doi: 10.3389/fpls.2015.00632

Figure 5.

Figure 5

Subcellular localization of PpPDI1 in P. parasitica. (A) Constructs used for expressing PpPDI1-EGFP in P. parasitica strain Pp016. In all constructs, transgenes are driven by the constitutive Bremia lactuca HSP70 promotor and HAM34 terminator. Plasmid pTHOE contains the full-length PpPDI1 ORF fused at the C-terminus with the EGFP. Plasmid TH210 contains the hygromycin-resistance gene (HPH) and is used as the selection marker in the co-transformation of P. parasitica. (B) Cytological characterization of P. parasitica transformant OE5 expressing translational fusion of PpPDI1 with EGFP (bar, 50 μm). Arrows indicate finger-like structures. (C) Expression analysis of PpPDI1 in transformants expressing PpPDI1-EGFP. Real-time RT-PCR evaluation of PpPDI1 expression used hyphal RNA from the vegetative hyphae of the recipient strain Pp016, the control transformant 1121, and three transformants with GFP signals (OE1, OE5, and OE12). The real-time RT-PCR experiments were repeated three times with independent RNA isolations. Bars represent the standard errors of three biological replicates. P. parasitica WS041 was used as a reference gene for quantification and normalization of PpPDI1 expression. (*P < 0.05; **P < 0.01).