Table 1.
Physiologic parameters of the in silico cell used in simulations. Note that the GCA uses a linearized reaction diffusion system leading to rates for σ l and P p.
| Physiological cell parameter values for GCA simulations | ||
|---|---|---|
| R | 10 μm | Cell radius |
| γ | 5 | Volume ratio between cytosol and ER |
| D Ca | 220 μm2/s | Diffusion coefficient of cytosolic Ca2+ |
| D E | 70 μm2/s | Diffusion coefficient of lumenal Ca2+ |
| D B | 95 μm2/s | Diffusion coefficient of mobile buffer |
| [Ca2+]0 | 55 nM | Cytosolic Ca2+ base level |
| [E]0 | 800 μM | Endoplasmic reticulum Ca2+ base level |
| [IP3] | 80 μM | IP3 concentration |
| [B]T | 52 μM | Total mobile buffer concentration (EGTA) |
| k B + | 0.3 (μMs)−1 | On rate of the mobile buffer |
| k B − | 1.5 s−1 | Dissociation rate of the mobile buffer |
| [B i]T | 30 μM | Total immobile buffer concentration |
| k Bi + | 600 (μMs)−1 | On rate of the immobile buffer |
| k Bi − | 100 s−1 | Dissociation rate of the immobile buffer |
| σ l | ≈0.01 s−1 | Leak flux constant given by varied P p, [Ca2+]0 and [E]0 |