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. 2015 Aug 18;5:13252. doi: 10.1038/srep13252

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(a) Segmented (green outlines) quantitative DHM phase contrast images of HCT-8 cells after 0 h, 24 h, and 48 h of incubation with cell culture medium (control) or OMVs LB226692 (containing 580 ng/ml of Stx2a). Scale bars are 10 μm. (b) Time-dependent gain of cellular dry mass calculated based on the growth area and the mean phase contrast (normalized to the respective dry mass at t₀). Quantitative phase images were calculated from digital off-axis holograms (acquired by custom built C++ software) utilizing custom built software developed in the PV-Wave 9.5 programming environment (www.roguewave.com) and analysed for cell covered area and average phase contrast by image segmentation with the cell profiler software (www.cellprofiler.org) from which the cellular dry mass was calculated.