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. 2015 Aug 18;5:13267. doi: 10.1038/srep13267

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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All images are of A549 human lung adenocarcinoma epithelial cells, stained with CellTrace CFSE. (a) Representative imaging flow cytometer reconstructed fluorescence images of cells flowing at a velocity of 0.2 m/s. Objective lens: 50X/0.55 (b) Representative wide-field fluorescence images of stationary A549 cells taken by fluorescence microscope. Objective lens: 100X/0.90. (c) Representative confocal microscope images of stationary A549 cells. Objective lens: 63X/1.30. Size of all image crops is 20 μm by 20 μm; scale bar is 5 μm.