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. 2015 Aug 18;5:13267. doi: 10.1038/srep13267

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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All images are of A549 cells flowing at a velocity of 0.2 m/s. (a) Histogram of the Feret’s diameters of the G1 (blue bar) and G2/M (red bar) arrested cells’ backscattering images. 300 cell images from each group are measured. The sketch shows the definition of the Feret’s diameter: the longest distance between any two points along the object’s boundary, also known as maximum caliper. (b) Two example backscattering images for each bin in the histogram. Hot colorbar represents intensity from 0 to 255. Sizes of all cell backscattering images are 20 μm by 20 μm; the scale bars is 5 μm.