Table 1. Comparison of cell morphological features between fluorescence images restored by our imaging flow cytometer prototype and taken by fluorescence microscope based on 100 cell images from each group.
Cell Images from | Area (um^2) |
Circularity |
Solidity |
Aspect Ratio |
||||
---|---|---|---|---|---|---|---|---|
Mean | St. D. | Mean | St. D. | Mean | St. D. | Mean | St. D. | |
Imaging Flow Cytometer | 126.77 | 3.199 | 0.82 | 0.028 | 0.96 | 0.007 | 1.52 | 0.088 |
Fluorescence Microscope | 128.74 | 4.511 | 0.87 | 0.048 | 0.96 | 0.013 | 1.08 | 0.064 |
Size is the above-background area of the cell image; circularity equals to 4πA/P2, where A is area, P is perimeter of the cell image; solidity is the ratio of area to convex area; aspect ratio is the ratio of major axis to minor axis of the cell image’s fitted ellipse.