Fig. 1.

8-Br-cAMP treatment increases miR-132 expression and steroidogenesis in mGCs. a Primary mGCs were isolated from 21-day-old mouse ovaries. FSHR protein of mGC was detected by immunofluorescence staining (FSHR: green, DAPI: blue). Nonspecific staining was visualized using PBS to replace either the primary or secondary antibodies. b Immunohistochemical detection of mGCs (indicated by asterisks) in 21-day-old mouse ovaries. Immunostaining of FSHR is shown in brown, and nonspecific staining was visualized using PBS. Hematoxylin nuclear staining is shown in blue. The concentrations of progesterone (c) and E₂ (d) per 10⁵ cells in culture medium were determined after 8-Br-cAMP treatment for 24 h and 48 h. e At the indicated time points after treatment with 8-Br-cAMP, the miR-132 levels in mGCs were determined using qRT-PCR. The results were normalized to U6 as an internal control. The results represent the mean +/- SEM of three independent experiments. Values with different superscripts (a, b) are significantly different (p < 0.05). **p < 0.01; ***p < 0.001, compared with the control (CTL). Scale bar: 100 μm