Fig. 3.

Downregulation of endogenous miR-132 inhibits E2 synthesis in mGCs. a mGCs were transfected with 100 nM miR-132 inhibitors or negative controls. Six hours after transfection, mGCs were cultured in the absence or presence of 8-Br-cAMP for another 48 h. The inhibition of endogenous miR-132 by specific inhibitors was validated by qRT-PCR. b The culture medium was collected for the measurement of E₂ levels after endogenous miR-132 had been knocked down. The results represent the mean +/- SEM of three independent experiments performed in triplicate. *, # p < 0.05; **p < 0.01; ## p < 0.005, compared with the negative control (NC)