Fig. 4.

Effects of miR-132 on Cyp11a1 and Cyp19a1 in mGCs. To examine the effect of miR-132 on Cyp11a1 and Cyp191a1 transcription, mGCs were transfected with 100 nM of miR-132 mimics (a) or 100 nM of miR-132 inhibitors in the absence (b) or presence of 8-Br-cAMP (c) for 48 h as indicated, or the corresponding negative controls. Total RNA was extracted from mGCs 48 h after transfection, and the mRNA expression levels of Cyp11a1 and Cyp19a1 were measured by real-time PCR. The results represent the mean +/-SEM of three independent experiments. # p < 0.05; **p < 0.01; ## p < 0.005, compared with the negative control (NC)