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. 2015 Aug 19;8:424. doi: 10.1186/s13071-015-1016-x

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© Wang et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Gene structure and expression of A. stephensi p38 MAPK. (a) The A. stephensi p38 (AsP38) MAPK gene includes 7 exons (black boxes; length in base pairs (bp) indicated), 6 introns, and spans 17,303 bp. (b) Total RNA was isolated and converted to cDNA from 30 dissected midguts from non-blood fed female A. stephensi. AsP38 MAPK-specific primers were used to amplify cDNA by conventional PCR; molecular standards (bp) are shown on left