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. 2015 Aug 19;8:424. doi: 10.1186/s13071-015-1016-x

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© Wang et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Inhibition of AsP38 MAPK altered antimicrobial peptide promoter activity in response to LPS or PGN stimulation in immortalized A. stephensi cells. ASE cells were transfected with an antimicrobial peptide luciferase promoter-reporter plasmid construct and stimulated 24 h later with 100 μg/ml LPS or 2 μg/ml PGN with 0.1 -10 μM BIRB796 or an equivalent volume of DMSO as a control. Graphs represent means ± SEMs of luciferase activity (relative light units, RLU) normalized to untreated controls, n = 3-7. Cecropin1 (top, white) or Gambicin (middle, gray), or Defensin1 (bottom, black) promoter activities are indicated. Data were analysed by Student’s t-test and significant p-values are shown. NS = not significant