Table 1.
Allelic Discrimination Assay Primers and Probes, by Single-Nucleotide Polymorphism (SNP) Site
| SNP Site (HBoV-1 Genome Positiona) | HBoV-1 Gene | Minor Allele Frequency, % | Samples Tested, No. | Forward Primer Sequence | Reverse Primer Sequence | Probe 1/Probe2b |
|---|---|---|---|---|---|---|
| 2856 | NP1 | 13 | 202 | GCATTGCTAGAGATGGTACTAATTCAATCT | GCCAGAACATATTTCTGTATTTTTGATCTAGTG | ATAACACTAGAGAACTATTG/ACACTAGAGAGCTATTG |
| 3937 | VP1 | 12 | 218 | GCTGGCGTTCACATCTTTTGT | TGACGTCCTCATCCCATGGA | TGCATTTGGGTAAGCAT/TGCATTTGGATAAGCAT |
| 4195 | VP1 | 27 | 198 | AGAACTGGTGAAAGCACTGAATTTACT | AACTCTTCTTGTTGGGACTTTTGGA | CTCTTTCATTGTTAACCC/CTCTTTCATTATTAACCC |
| 4243 | VP1 | 43 | 211 | GAAAGAGCATACATTCCTCCTGGA | CTGTGCTTCCGTTTTGTCTTATGT | CTTGTTGGGACTTTTG/CTTGTTGGAACTTTTG |
| 4756 | VP1 | 38 | 201 | GGGACAGATTTCCTATCACCAGAGA | AGTGCCTGGAGGATGATCCAT | TGATGGATCAATTGCA/TTTGATGGATCCATTGCA |
| 4813 | VP1 | 37 | 187 | GGATCATCCTCCAGGCACTATTTT | ACAGCTGACTTGTCCAGTACAGTAT | TTCCAGTACCAACTGC/CCAGTTCCAACTGC |
| 4876 | VP1 | 15 | 208 | GGATCATCCTCCAGGCACTATTTT | CCAGTTCTTTGTTGCGTATCTTTCT | CAGCTGTGAAATTGTA/CAGCTGTGAGATTGTA |
| 4888 | VP1 | 38 | 220 | GTACTGGACAAGTCAGCTGTGA | CTGGACGCCAGTTCTTTGTTG | CGTATCTTTCTACTTCCC/CGTATCTTTCTACCTCCC |
Abbreviations: HBoV-1, human bocavirus 1; SNP, single-nucleotide polymorphism.
a Nucleotide position references GenBank accession number DQ000496.
b SNP alleles are underlined.