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. 2015 Aug 17;210(4):553–564. doi: 10.1083/jcb.201502046

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© 2015 Lawrimore et al.

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Figure 4. — Tension along the spindle axis promotes microtubule growth. TetO array extension and kMT length in unreplicated, dicentric plasmids versus replicated, monocentric plasmids. Image of metaphase yeast containing an unreplicated, dicentric plasmid (A) and a replicated, monocentric plasmid (B). Schematic of a spindle containing an unreplicated, dicentric plasmid (C) and a replicated, monocentric plasmid (D). SPBs are shown in red, kinetochores are shown in blue, and DNA is shown in green. Tet array extension and kMT length versus spindle length for unreplicated, dicentric plasmids (E) and for replicated, monocentric plasmids (F). Bar, 0.5 µm. Error bars are standard deviation. The data shown are from multiple experiments (dicentric, n = 43 arrays; replicated monocentric, n = 54).