Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 17;210(4):553–564. doi: 10.1083/jcb.201502046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Lawrimore et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Higher DNA binding protein turnover proximal to the spindle axis. FRAP analysis of TetR-GFP and condensin. (A) Representative images of dicentric plasmids pre, post, and 2–4 min after photobleaching. Blue arrowhead marks the bleached TetR-GFP spot. SPBs are indicated by white arrows. Bar, 0.5 µm. (B) Images of condensin pre, post, and 5 min after photobleaching. Blue arrowhead indicates the bleached condensin bound to pericentric DNA. Red arrow indicates condensin bound to the rDNA locus. Only pericentric condensin was bleached in the cell shown. Bar, 0.5 µm. (C) The percentage of cells showing recovery of condensin bound to the pericentric and rDNA loci in WT and mcm21Δ cells 10 min after bleaching. Recovery threshold is 30% of the original corrected signal. Each dataset summarizes multiple experiments (WT rDNA, n = 14; mcm21Δ, n = 13; WT pericentric, n = 12; mcm21Δ pericentric, n = 12 cells).

HHS Vulnerability Disclosure