Table 1. Radii of confinement calculated for untreated and treated cells.

Metric	Equation	Calculation	LacO array distance from centromere
			240 kb untreated	240 kb azide	6.8 kb untreated	6.8 kb benomyl	6.8 kb azide
			Sample size (foci)
			40	41	41	41	37
Rc (nm)	$=\frac{5}{4}\mathrm{ }\times \sqrt{MS{D}_{plateau}}$	Plateau (individual cell)	664 ± 243	187 ± 68.3	388 ± 152	306 ± 118	89.6 ± 25.6
	$=\frac{5}{4}\mathrm{ }\times \sqrt{\mathrm{ }(2{\sigma }^2\mathrm{ }+\mathrm{ }\Delta r_0^2)}$	σ (individual cell)	593 ± 170	165 ± 56.7	350 ± 112	280 ± 84	84.0 ± 22.9
		σ (entire data set)	609	172	363	289	86
Effective spring constant (pN/nm)	$=\mathrm{ }\frac{2k_{b}T}{MS{D}_{plateau}-\mathrm{ }\Delta r_0^2}$	Plateau (individual cell)	9.4 × 10−5 ± 1.0 × 10−4	1.22 × 10−3 ± 1.7 × 10−3	2.4 × 10−4 ± 2.2 × 10−4	4.4 × 10−4 ± 3.9 × 10−4	4.0 × 10−3 ± 4.0 × 10−3
	$=\mathrm{ }\frac{k_{B}T}{{\sigma }^2}$	σ (individual cell)	9.1 × 10−5 ± 5.7 × 10−5	1.54 × 10−3 ± 1.8 × 10−3	2.9 × 10−4 ± 2.2 × 10−4	4.4 × 10−4 ± 3.4 × 10−4	4.7 × 10−3 ± 3.4 × 10−3
		σ (entire data set)	6.9 × 10−5	8.6 × 10−4	1.9 × 10−4	3.1 × 10−4	4.7 × 10−3