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. 2015 Aug 17;210(4):613–627. doi: 10.1083/jcb.201501073

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© 2015 Zhao et al.

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Figure 6. — miR-7 regulates RELA activation by modulating IKKε. (A) Western blot analysis of RELA, p-RELA, IκBα, and p-IκBα levels in GES cells after the indicated treatment. LPS, lipopolysaccharide. (B) Predicted duplex sequences between CHUP and IKBKE 3′-UTRs and miR-7. (C) Western blot analysis of IKKα and IKKε levels in the indicated cells after miR-7 overexpression and inhibition. (D) Luciferase activity derived from the indicated 3′-UTR reporter constructs after cotransfection into BGC823 cells with miR-7, anti–miR-7, or their negative controls. Results show the means ± SD (error bars) of three experiments performed in triplicates. (E) The changes in IKKε, IκBα, p-IκBα, RELA, and p-RELA levels in GES cells when cotransfected the IKKε plasmids containing the wild-type or mutant 3′-UTR, along with miR-7 or control, were detected by Western blotting. *, P < 0.05; N.S., not significant (P > 0.05).