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. 2015 Aug 13;3:e1147. doi: 10.7717/peerj.1147

Table 3. Average DNA concentration (ng/μl) of whole and cut nymphal and adult female blacklegged ticks.

Average and standard deviation of the DNA concentration values determined using the Qubit HS dsDNA Assay. Unless otherwise indicated, samples were stored in 70% v/v ethanol. Three single-tick measurements were included in each treatment. All values listed as <0.0005 ng/μl indicate a reading of “too low” from the Qubit fluorometer.

Method Life stage Nymphs Adult females
Bisected Whole Quadrisected Whole
QIAGEN Average 1.70 Table S1 9.41 Table S1
SD 0.640 1.63
Thermo Average 1.68 Table S1 9.77 Table S1
SD 0.11 2.35
MoBio Average 0.070 0.0373 4.51 0.119
SD 0.0585 0.0457 0.492 0.0133
Zymo Average <0.0005 <0.0005 3.14 0.0146
SD 0.00 0.00 1.44 0.00341
NH4OH Average 0.677 Table S1 0.0240 Table S1
SD 0.086 0.0143