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. Author manuscript; available in PMC: 2015 Aug 18.
Published in final edited form as: Dev Biol. 2009 Nov 13;337(2):396–404. doi: 10.1016/j.ydbio.2009.11.015

Fig. 1.

Fig. 1

Foxj3 is expressed in C2C12 myoblasts. Semiquantitative RT-PCR analysis of transcript expression during C2C12 differentiation from myoblasts (50% or 90% confluency, cultured in growth medium or GM) to myotubes (day 1 or d1 to day 4 or d4, cultured in differentiation medium or DM) demonstrates that Foxj3 is expressed in myoblasts prior to myoglobin (Mb) expression. Rn18s was used as a loading control. (−) represents a negative control (lacking reverse transcriptase).