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. Author manuscript; available in PMC: 2015 Aug 18.
Published in final edited form as: Dev Biol. 2009 Nov 13;337(2):396–404. doi: 10.1016/j.ydbio.2009.11.015

Fig. 4.

Fig. 4

shRNA knockdown of endogenous Foxj3 results in increased myoblast proliferation. (A) Real-time PCR confirming knockdown of endogenous Foxj3 transcript in cells transfected with shRNA directed against Foxj3 (n=3 replicates; *p<0.05). (B) Phase microscopy of C2C12 myoblasts 48 h post-transfection of both control (pRS; empty vector) and shRNA directed against mouse Foxj3; shRNA #1 and #2. Note increased number of myoblasts with knockdown of Foxj3. (C) Table summarizing the results of the FACS histogram profiles of the C2C12 myoblasts transfected with control and shRNA Foxj3 vectors (n=3 replicates; *p<0.03; **p<0.003).