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. Author manuscript; available in PMC: 2015 Aug 18.
Published in final edited form as: Steroids. 2013 Nov 20;81:17–25. doi: 10.1016/j.steroids.2013.11.009

Fig. 1.

Fig. 1

Expression of membrane progestin receptor α (mPRα, Paqr7) and mPRβ (Paqr8) transcripts in proestrus C57/BL6 mouse peripheral tissues and brain regions, as analyzed by RT-PCR. P: prefrontal cortex; Hi; hippocampus; A: amygdala; Hy: hypothalamus; M: midbrain; B: brain; B S: spleen; H: heart; Lg: lungs; K: kidney; Lv: liver; and In: intestines. Samples were prepared for all tissues samples using same procedure with (+, indicated in Panel B) or without Superscript III (−, indicated in Panel B). Forward and reverse PCR primers were specifically designed to be located in two different exons. Specific PCR products amplified from the transcripts with expected size were observed (525 bp for mPRα, and 328 bp for mPRβ). There was no evidence of genomic DNA contamination.