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. 2015 Apr 1;4(1):229–241. doi: 10.1089/biores.2015.0014

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© Eamon J. Sheehy et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 2. — MSC-encapsulated alginate hydrogels were fabricated in the shape of the TMJ condyle. Constructs were maintained in chondrogenic culture conditions for a period of 5 weeks, followed by maintenance in hypertrophic culture conditions for an additional 3 weeks. (a) Macroscopic image of TMJ construct at week 8. Scale bar is 2 mm. (b) Aldehyde fuchsin/alcian blue, (c) alizarin red, and (d) picrosirius red staining of TMJ constructs at week 8 to assess sGAG, calcium, and collagen spatial accumulation, respectively. Scale bars are 500 μm. TMJ, temporomandibular joint.