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. 2015 Apr 1;4(1):229–241. doi: 10.1089/biores.2015.0014

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© Eamon J. Sheehy et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 3. — Anatomically shaped alginate phalanx constructs, cultured in vitro for 5 weeks, were implanted subcutaneously into nude mice and harvested 8 weeks postimplantation. (a) Macroscopic image of construct. (b) Picrosirius red staining of construct. (c) Aldehyde fuchsin/alcian blue staining of construct. Insets show collagen type II (top), collagen type X (center), and collagen type I (bottom) staining. (d) H&E staining of construct. (e) μCT image of whole construct. (f) μCT image of center section of construct corresponding to a thickness of 300 μm. Arrows in (d) indicate blood vessel structures. Main image scale bars are 2 mm. Inset scale bars in (c) are 250 μm. Inset scale bars in (d) are 100 μm. H&E, hematoxylin and eosin; μCT, microcomputed tomography.