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. 2015 Aug 12;10:5123–5137. doi: 10.2147/IJN.S87011

Figure 5.

Figure 5

Flow cytometry assay of DOX fluorescence intensities associated with ASGPR-positive HepG2 (A), BEL7402 (B), SMMC7721 (C), and ASGPR-negative NIH 3T3 (D) cells.

Notes: Cells were incubated with DOX-loaded PLS (blue lines) and Lf-PLS (orange lines) (DOX concentration 40 μg/mL) for 4 hours at 37°C, respectively. The untreated cells (red lines) were used as the blank controls. Data represent mean ± SD (n=3). *P<0.05 versus PLS group.

Abbreviations: ASGPR, asialoglycoprotein receptor; DOX, doxorubicin; FL2-H, fluorescence intensity of DOX; Lf-PLS, lactoferrin-modified PEGylated liposome; PEG, polyethylene glycol; PLS, PEGylated liposome; SD, standard deviation.