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. 2015 Jun 24;107(4):466–477. doi: 10.1093/cvr/cvv184

Figure 2.

Figure 2

Relative mRNA expression of AnkB-188 and AnkB-212 in human and mouse cardiac tissues. (A) Exon–exon boundary spanning primers were used to PCR amplify alternative splice junctions unique to each isoform (AnkB-188: junction 45/51, AnkB-212: junction 50/51). (B) Relative mRNA expression of alternative splice junctions in AnkB-188 and AnkB-212 was measured in cardiac tissues from three human hearts. Expression of each splice junction was normalized to the expression of exon junction 31/32 (presumably expressed in all ANK2 transcripts and set to 100%). (C) Relative mRNA expression of alternative splice junctions in AnkB-188 and AnkB-212 was measured in cardiac tissues from three mouse hearts. For (B and C), samples were repeated in triplicate (n = 3) and experiments were repeated three times.