Quartz crystal microbalance with dissipation. Effects of 23 nm polystyrene nanoparticles on platelet microaggregation.
Notes: (A) Quantitative analysis of the effects of perfusion of PPP and PRP in the presence or absence of 23 nm polystyrene nanoparticles and modulators of platelet function on frequency and energy dissipation. The data are presented as the mean ± standard deviation. **P<0.01; ***P<0.001 versus PRP. ^P<0.05; ^^P<0.01; ^^^P<0.001 versus PRP +23 nm polystyrene nanoparticles. (B) Representative micrographs of the surface of fibrinogen-coated polystyrene-quartz crystals as viewed by optical microscopy showing platelet aggregates following perfusion with PRP in the absence and presence of 23 nm polystyrene nanoparticles and ASA as an example of a pharmacological modulator of platelet function. Scale bar 20 μm.
Abbreviations: PRP, platelet-rich plasma; PPP, platelet-poor plasma; ASA, acetylsalicylic acid; Phen, phenanthroline; GSNO, S-nitroso-glutathione; Si, silica; SNAP, S-nitroso-N-acetyl-DL-penicillamine; Poly, polystyrene.