Figure 6.

CTN06 as a chemo-sensitizer. (a) Comparison of CTN06 with other kinase inhibitors. PC3 cells were seeded at 5000 cells/well in 96-well plates overnight and treated with CTN06 or the other kinase inhibitors including AZD0530, sorafenib, AG1478, 3ATA and LMF-A13 at 2 μM. The cell viability was measured using MTT assay after 72 h. (b) Growth inhibition of CTN06 and in combination with 10 μM chloroquine (CQ) or 2 ng/ml docetaxel (DTX) to PC3 human prostate cancer cells. Cells were seeded at 5000 cells/well in 96-well plate overnight and pretreated with the corresponding co-treatments for 1 h, then treated with 0.25 μM CTN06. The cell viability was measured using MTT assay after 72 h. (c) The apoptotic effect of CTN06 in combination with CQ to PC3 cells was further measured using Annexin-V and PI staining and flow cytometry. Columns, mean; bars, standard deviation, n=3