Figure 4.

MUC1 is a bona fide target of miR455-3P in BeWo cells. (a) Expression of potential miR455 target mRNAs in FSK-treated BeWo cells. Expression of the indicated potential miR455 target mRNAs was analyzed by qRT-PCR. Values were normalized to RPLP0 and displayed relative to DMSO-treated cells. (b) MUC1 protein levels are reduced by FSK treatment. MUC1 and TBP protein levels were analyzed by western blotting 48 h after treatment. (c and d) MUC1 is repressed by miR455-3P but not miR455-5P. BeWo cells were transfected with synthetic miRNAs at two concentrations (5 and 20 nM). RNA and protein samples were harvested 48 h post-transfection. MUC1 mRNA and protein levels were determined by qRT-PCR (c) and western blotting (d), respectively. One western blot representative of a transfection with 20 nM synthetic miRNA is presented in d. TBP served as a loading control