Fig. 7.
X-band CW-EPR spectra of the reaction products of H2O2 and bCcO trapped via rapid freeze-quench at pH 6 (a) and pH 8 (b). The bCcO (160 µM) was reacted with 2.2 M H2O2 and trapped at 50 µs. The buffers were: 200 mM BIS–TRIS + 0.2% w/v DM at pH 6, 200 mM HEPES + 0.2% w/v DM at pH 8. The EPR conditions were the same as in Fig. 3.