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. 2015 Aug 18;10(8):e0135314. doi: 10.1371/journal.pone.0135314

Fig 3. Verification of differential expression of the key proteins identified by proteomics.

Fig 3

(A) Relative expression of four differentially expressed proteins—deoxycytidine kinase (dCK), phoshatase SHP-1 (alias PTPN6), Bcl-2 and phoshoserine aminotransferase (PSAT-1)–was determined by Western Blotting using specific antibodies in Mino and Mino/FR cells. GAPDH was used as a loading control. (B) Relative expression of two surface CD markers (CD20 and CD38) determined by flow cytometry using specific antibodies. Open histograms represent Mino/FR cells, full histograms show Mino cells. Histograms demonstrate approximately 2-fold decreased expression of CD20 and CD38 in Mino/FR cells as indicated by decreased median fluorescence intensity.