Fig 3. MiR-200c enhances immune suppressive function of MDSCs.

(A, B, C and D) Production of ROS (A), H₂O₂ (B), NO- (C) and arginase activity (D) in differently transfected cells. BM cells were transfected with miR-200c mimics (miR-200cM), miR-200c inhibitors (Anti-200cI), or control oligoes (Ctr. oligoes) and then further cultured in the presence of GM-CSF and IL-6 for 4 days. ROS, H₂O₂, NO- and arginase activity were analyzed as described in Materials and Methods. (E) Suppressive function of differently treated MDSCs. CFDA-SE-labeled CD4⁺ or CD8⁺T cells were stimulated with anti-CD3 mAbs in the presence of MDSCs, which were pre-treated with either miR-200c mimics (miR-200cM), anti-200c inhibitors (miR-200cI) or control oligoes (Ctr. ologoes). Numbers % indicated the percentages of CD8⁺ or CD4⁺ proliferative cells. Positive Ctr., positive control without MDSCs. Data are a representative of three independent experiments. *, p<0.05; **, P<0.01.