Table 2. Oligonucleotides used.
sequence (5´-3´) | |
---|---|
dhc1AaXbaI | AGT CTC TAG AGC CAC ACC GAA TCG CCA GCA AGT C |
dhc1AbXbaI | CCA TTC TAG AGG GGG AAG CAG CGC GAG CAG GTA |
dhc1Ba | ACG CGC ATA GGG GCA CCG ACA A |
dhc1Bb | GCA AGC GCC ATC CCC AGC AGT |
Dyn1* | CCNGCNGGNACNGGNAARACNGA |
Dyn3* | ARNCCRAARTCRTARTG |
Dyn4* | TGYTTYGAYGARTTYAA |
dyn2-1up* | CAGCAGAAYGTBGABATHCC |
dyn2-8low* | GCCCAKATKATBGCBCC |
dhcClaI | CCATCGATGCACCAGCTCCGTGAT |
dhcKpnI | GGGGTACCGAGTGGGAGACGACGC |
E6-1rev | GCTTCGCCTCCTTGATGA |
dyn2-2for | CATGCCTTGGTCTTCTTCTTG |
kin2-1fw | AACTGCACGCTTTTCGCTTACG |
kin2-1rev | CCGGCGTTAGAGGATGGG |
kin14start-for | ATGGACGCCGAGCGCGCCAA |
kin14start-rev | CTACTTCACTACCCTCCTCGCCG |
*degenerated primers