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. 2015 Aug 18;11(8):e1005102. doi: 10.1371/journal.ppat.1005102

Fig 4. Tax redistributes RNF8 from the nucleus to the cytoplasm.

Fig 4

(A) RNF8 is localized to the cytoplasm when Tax is present. HeLa-G cells were transduced with Ad-Tax at an MOI of 0.5 for 48 hours. HeLa-G cells contain a Tax-inducible GFP cassette and turn brightly green upon Tax expression as indicated by white arrows. The cells were then fixed, permeabilized and stained with an antibody for RNF8 (Red) and DAPI (Blue). (B) HeLa cells were transduced with Ad-Tax or Ad-tTa for 48 hours and fractionated following manufacturer’s protocol. The cytosolic and nuclear fractions were immunoblotted for the indicated proteins. (C) Much RNF8 is localized in the cytoplasm of Jurkat and HTLV-1-transformed MT4 T cell lines. Cells were fixed, permeabilized and stained with an antibody for RNF8 (Red) and DAPI (Blue). (D) Jurkat and MT4 cells were harvested and fractionated following manufacturer’s protocol. The cytosolic and nuclear fractions were immunoblotted for the indicated proteins. HDAC1 and GAPDH are used as nuclear and cytosolic protein control respectively for both (B) and (D).