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. Author manuscript; available in PMC: 2015 Aug 18.
Published in final edited form as: Curr Protoc Microbiol. 2011 May;0 2:Unit–2C.4. doi: 10.1002/9780471729259.mc02c04s21

Fig. 6.

Fig. 6

Steps for collecting luminescent data for 3D DLIT reconstruction. (a) IVIS control panel showing the image sequence editor and the Imaging wizard. (b) Imaging wizard bioluminescence options. (c) Living Image Tool Palette showing the Surface topography selection. (d) Surface topography analysis panel showing the Crop area of interest. (e) Surface topography analysis panel showing the appropriate threshold selection. (f) Living Image reconstruction panel showing the 3D surface generation. (g) Living Image Tool Palette showing the DLIT 3D reconstruction settings. (h) Living Image reconstruction panel showing the data preview.