A) Flexural rigidities of bare, MVT−, VT−, and VT+MVT-decorated actin filaments (All binding densities = 0.9). Solid lines represent best fits of the two-dimensional average cosine angular correlation, <C(s)>, along filament segment lengths (x) (<C(s)> = A*exp(−x/2 Lp)). B) Bending Lp calculated from the best fit of angular correlation data as shown in (A). Lp of bare actin: 8.35 ± 0.17μm, MVT-decorated actin: 3.92 ± 0.46μm, VT-decorated actin: 15.5 ± 1.4μm, MVT/VT-decorated actin: 17.7 ± 1.2μm. Uncertainty corresponds to the standard error of the fit, n = 50-200 filaments/condition.