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. Author manuscript; available in PMC: 2016 Aug 28.
Published in final edited form as: J Mol Biol. 2015 Jul 10;427(17):2782–2798. doi: 10.1016/j.jmb.2015.07.005

Figure 8. MVT reduces the F-actin bundling activity of VT.

Figure 8

F-actin and vinculins were mixed at the indicated molar ratios and incubated for 1 hour at 24 °C. Samples were subjected to differential centrifugation to separate fractions with actin bundles (LP, low speed pellet) or filaments (HP, high speed pellet). Unbound vinculins were in the high speed supernatant fraction (HS). A) Fractions were analyzed on SDS-PAGEs and B) subjected to densitometry analysis to determine the percentage of actin filaments in bundles and the binding densities of VT and MVT on F-actin (sum of LP+HP). Increasing molar ratios of MVT to VT were found to decrease VT’s bundling activity as seen in Figure 7.