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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mucosal Immunol. 2015 Apr 22;8(5):1166–1179. doi: 10.1038/mi.2015.30

Figure 7. Breast milk restores enterocyte proliferation via EGFR and GSK3β.

Figure 7

A–C: Representative confocal micrographs of either wild-type IEC-6 cells (Ai–v) or EGFR-kd IEC-6 (Bi–v) or GSK3β-kd IEC-6 cells (Ci–iii), treated as indicated, then stained for the proliferation marker bromodeoxyuridine (BrdU, red, arrows) and DAPI (blue). D: Quantification of the BrdU positive cells per high power field (hpf) in wild-type IEC-6 cells (i) or EGFR-kd IEC-6 (ii) or GSK3β-kd IEC-6 cells (iii). *p<0.05 versus control (Ctrl, white bar), **p<0.05 versus LPS, ***p<0.05 versus LPS + breast milk (BM), ****p<0.05 versus LPS + IBM. Results representative of three separate experiments with over 50 high power fields per group. Size bar = 10μm. Data are mean±SEM. Arrows delineate proliferative cells.